Genetic analyses of Coprinus cinereus strains derived through intraspecific protoplast fusion.
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چکیده
منابع مشابه
Enhanced intraspecific protoplast fusion in yeast.
Intraspecific hybrid production from the polyethylene glycol induced fusion of yeast protoplasts was greatly increased when calcium propionate was included as the source of the requisite Ca2+. The use of calcium propionate, as opposed to the more commonly employed calcium chloride, resulted in substantially greater yields of hybrids from intraspecific fusions of protoplasts of Saccharomyces cer...
متن کاملCorrelation of genetic and physical maps at the A mating-type locus of Coprinus cinereus.
The A mating type locus of Coprinus cinereus is remarkable for its extreme diversity, with over 100 different alleles in natural populations. Classical genetic studies have demonstrated that this hypervariability arises in part from recombination between two subloci of A, alpha and beta, although more recent population genetic data have indicated a third segregating sublocus. In this study, we ...
متن کاملThe origin of multiple B mating specificities in Coprinus cinereus.
Mushrooms, such as Coprinus cinereus, possess large families of pheromones and G-protein-coupled receptors that are sequestered at the B mating-type locus and whose function is to confer vast numbers of different mating types. This ability results from complex patterns of cognate and noncognate pheromone/receptor pairings, which potentially offer a unique insight into the molecular interaction ...
متن کاملDe novo methylation of repeated sequences in Coprinus cinereus.
We have examined the stability of duplicated DNA sequences in the sexual phase of the life cycle of the basidiomycete fungus, Coprinus cinereus. We observed premeiotic de novo methylation in haploid nuclei containing either a triplication, a tandem duplication, or an ectopic duplication. Methylation changes were not observed in unique sequences. Repeated sequences underwent methylation changes ...
متن کاملSome characteristics of extracellular proteinases from Coprinus cinereus
Spent culture medium contained five electrophoretically distinct proteolytic activities when Coprinus c;llereus was grown in culture medium which contained protein as sole source of nitrogen. The enzymes were separated by ultrafiltration, gel filtration and ionexchange chromatography into two groups designated EL and AD fractions. The EL fraction passed through a DE-52 column with eluant, and c...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1988
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.52.281